C h e m G e n e s   I n d i a

Dark Quencher-534 & Dark Quencher-579 Amidites & Supports

Dark Quencher-534 & Dark Quencher-579 Amidites & Supports


ChemGenes India introduces Dark Quencher-534 & Dark Quencher-579 Amidites & Supports.

Dark Quencher-534 Phopophoramidite & Dark Quencher-579 Phopophoramidite are a non-fluorescent chromophore, and extensively used as the 3'-quencher moiety in a variety of FRET (Fluorescence Resonance Energy Transfer) in DNA detection probes. Such probes are primarily used in nucleic acid assays.
  1. Advantage

    The advantages of using a Dark Quencher in a FRET probe are

    • Low background fluorescence & higher dynamic range,
    • Responsiveness to multiplex assays - because of a dark quencher having no secondary fluorescence.
    • Comfort of synthesis of FRET probes with a Dark Quencher - because dark quenchers being resistant to degradation throughout the oligo deprotection step.
  2. Storage and handling
    Shipping conditions: Cold Box Storage conditions: -15 to -30 °C, Light sensitive material.
Product Properties
Dark Quencher – 534 Phosporamidites
Dark_Quencher_–_534_Phosporamidites Chemical Name 4'-(2-Nitro-4-toluyldiazo)-2'-methoxy-5'-methyl-azobenzene-4''-(N-ethyl)-N-ethyl-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite
Chemical Formula C34H45N8O5P
Molecular Weight 676.75
Appearance Dark Red Solid
Absorption range Dark Quencher– 534 Phosporamidites: 480~580 nm
Pack Size 100mg, 250mg, 500mg, 1.0g
Catalog CGIDQ-534-01 for pack size 100mg
CGIDQ-534-25 for pack size 250mg
CGIDQ-534-05 for pack size 500mg
CGIDQ-534-10 for pack size 1.0g
Dark Quencher-579 Phopophoramidite
Dark_Quencher_–_579_Phosporamidites Chemical Name 4'-(4-Nitro-phenyldiazo)-2'-methoxy-5'-methoxy-azobenzene-4''-(N-ethyl)-N-ethyl-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite
Chemical Formula C33H43N8O6P
Molecular Weight 678.72
Appearance Dark Purple Solid
Absorption range Dark Quencher-579 Phopophoramidite: 550~650 nm
Pack Size 100mg, 250mg, 500mg, 1.0g
Catalog CGIDQ-579-01 for pack size 100mg
CGIDQ-579-25 for pack size 250mg
CGIDQ-579-05 for pack size 500mg
CGIDQ-579-10 for pack size 1.0g
Dark Quencher-534 CPG
Dark_Quencher_–_534_CPG Chemical Name 4'-(2-Nitro-4-toluyldiazo)-2'-methoxy-5'-methyl-azobenzene-4''-(N-ethyl-2-O-(4,4'-dimethoxytrityl))-N-ethyl-2-O-glycolate-CPG
Appearance Dark Purple Solid
Absorption range 480 to 580 nm
Pore Size 500Å,1000Å, 2000 Å
Loading 500Å : 60~70µmol/g
1000Å : 30~40µmol/g
2000Å : 20~30µmol/g
500 Å CPG is suitable for high production applications (≤30mers).
1000Å CPG is suitable for highly modified oligonucleotides (>20mers).
Purity >90%
Stability 1 Year
Pack Size 1.0µm x1unit & 1.0µm x5 units
Catalog CGIDQCPG-534-01-1 for 1.0µm x1unit
CGIDQCPG-534-01-5 for 1.0µm x5 units
Dark Quencher-579 CPG
Dark_Quencher_–_579_CPG Chemical Name 4'-(4-Nitro-phenyldiazo)-2'-methoxy-5'-methoxy-azobenzene-4''-(N-ethyl-2-O-(4,4'-dimethoxytrityl))-N-ethyl-2-O-glycolate-CPG
Appearance Purple Powder
Absorption range 480 to 580 nm
Pore Size 500Å ,1000Å, 2000 Å
Loading 500Å : 60~70µmol/g
1000Å : 30~40µmol/g
2000Å : 20~30µmol/g
500 Å CPG is suitable for high production applications (≤30mers).
1000Å CPG is suitable for highly modified oligonucleotides (>20mers).
Purity >90%
Stability 1 Year
Pack Size 10µm x 1 Unit
10µm x 2 Unit
Catalog CGIDQCPG-579-10-1 for 10µm x 1 Unit
CGIDQCPG-579-10-2 for 10µm x 2 Unit