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Dark Quencher-534 & Dark Quencher-579 Amidites & Supports
Dark Quencher-534 & Dark Quencher-579 Amidites & Supports
ChemGenes India introduces
Dark Quencher-534 & Dark Quencher-579 Amidites & Supports.
Dark Quencher-534 Phopophoramidite & Dark Quencher-579 Phopophoramidite
are a non-fluorescent chromophore, and extensively used as the 3'-quencher moiety in a variety of FRET (Fluorescence Resonance Energy Transfer) in DNA detection probes. Such probes are primarily used in nucleic acid assays.
Advantage
The advantages of using a Dark Quencher in a FRET probe are
Low background fluorescence & higher dynamic range,
Responsiveness to multiplex assays - because of a dark quencher having no secondary fluorescence.
Comfort of synthesis of FRET probes with a Dark Quencher - because dark quenchers being resistant to degradation throughout the oligo deprotection step.
Storage and handling
Shipping conditions: Cold Box Storage conditions: -15 to -30 °C, Light sensitive material.
Product
Properties
Dark Quencher – 534 Phosporamidites
Chemical Name
4'-(2-Nitro-4-toluyldiazo)-2'-methoxy-5'-methyl-azobenzene-4''-(N-ethyl)-N-ethyl-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite
Chemical Formula
C
34
H
45
N
8
O
5
P
Molecular Weight
676.75
Appearance
Dark Red Solid
Absorption range
Dark Quencher– 534 Phosporamidites: 480~580 nm
Pack Size
100mg, 250mg, 500mg, 1.0g
Catalog
CGIDQ-534-01 for pack size 100mg
CGIDQ-534-25 for pack size 250mg
CGIDQ-534-05 for pack size 500mg
CGIDQ-534-10 for pack size 1.0g
Dark Quencher-579 Phopophoramidite
Chemical Name
4'-(4-Nitro-phenyldiazo)-2'-methoxy-5'-methoxy-azobenzene-4''-(N-ethyl)-N-ethyl-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite
Chemical Formula
C
33
H
43
N
8
O
6
P
Molecular Weight
678.72
Appearance
Dark Purple Solid
Absorption range
Dark Quencher-579 Phopophoramidite: 550~650 nm
Pack Size
100mg, 250mg, 500mg, 1.0g
Catalog
CGIDQ-579-01 for pack size 100mg
CGIDQ-579-25 for pack size 250mg
CGIDQ-579-05 for pack size 500mg
CGIDQ-579-10 for pack size 1.0g
Dark Quencher-534 CPG
Chemical Name
4'-(2-Nitro-4-toluyldiazo)-2'-methoxy-5'-methyl-azobenzene-4''-(N-ethyl-2-O-(4,4'-dimethoxytrityl))-N-ethyl-2-O-glycolate-CPG
Appearance
Dark Purple Solid
Absorption range
480 to 580 nm
Pore Size
500Å,1000Å, 2000 Å
Loading
500Å : 60~70µmol/g
1000Å : 30~40µmol/g
2000Å : 20~30µmol/g
500 Å CPG is suitable for high production applications (≤30mers).
1000Å CPG is suitable for highly modified oligonucleotides (>20mers).
Purity
>90%
Stability
1 Year
Pack Size
1.0µm x1unit & 1.0µm x5 units
Catalog
CGIDQCPG-534-01-1 for 1.0µm x1unit
CGIDQCPG-534-01-5 for 1.0µm x5 units
Dark Quencher-579 CPG
Chemical Name
4'-(4-Nitro-phenyldiazo)-2'-methoxy-5'-methoxy-azobenzene-4''-(N-ethyl-2-O-(4,4'-dimethoxytrityl))-N-ethyl-2-O-glycolate-CPG
Appearance
Purple Powder
Absorption range
480 to 580 nm
Pore Size
500Å ,1000Å, 2000 Å
Loading
500Å : 60~70µmol/g
1000Å : 30~40µmol/g
2000Å : 20~30µmol/g
500 Å CPG is suitable for high production applications (≤30mers).
1000Å CPG is suitable for highly modified oligonucleotides (>20mers).
Purity
>90%
Stability
1 Year
Pack Size
10µm x 1 Unit
10µm x 2 Unit
Catalog
CGIDQCPG-579-10-1 for 10µm x 1 Unit
CGIDQCPG-579-10-2 for 10µm x 2 Unit
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